Nano suspensions are colloidal dispersions containing nanoparticles of drug that have been stabilised with surfactants
and are used in the manufacture of nanomedicine. Additionally referred to as biphasic systems, they consist of pure drug
particles dispersed in an aqueous medium with a diameter of less than 1 micron and a suspended particle with a diameter of less
than 1 micron. The majority of drugs used today are lipophilic, and many are insoluble in water owing to their functional
groups, particle size, chemical composition, and other variables, notably anti-diabetic pharmaceuticals such as Nateglinide.
Thus, the purpose of this work is to create nano-suspensions of nateglinide utilising poloxamer as a polymer and to evaluate
their increased bioavailability. Physical criteria such as drug entrapment efficiency, drug content, yield, surface morphology,
and in vitro drug release studies were tested on the produced formulations. In vitro testing in a PH 1.2 phosphate buffer
revealed that the nanosuspension formulation provided more drug release than the pure drug. As a result, nanosuspensions may
represent a viable alternative to traditional delivery systems for medications with low water solubility, with the potential to
enhance their biopharmaceutical performance

Standardization of herbal drug formulation is an essential process to assess the quality and purity of product. The Indian
subcontinent is enriched by a variety of flora-both medical and aromatic plants. Herbal medicines are now in high demand in
the advancing world for primary health care not because they are in-expansive but also for the superior cultutral acceptability,
better compatability with the human body and minimal side effects. The high usage of herbal drugs by the people is the driving
force to evaluate the health claim of these products. Nilavembu churna - a poly herbal formulation, is a decoction concentrate
commonly used in siddha medicine for the treatment of dengue fever. Synonym for nilavembu- Green chirata, Creat, King of
bitter, kirayat, etc., This plant has got a enormous therpeutic efficiency in treating different type of sickness in human body.
Such as skin disorder, hepato biliary illness, diabetes mellitus and others. In this study, evaluating the nilavembu churna
product by 1)Organoleptic parameters - Appearance, Colour, Odour, Taste, texture and 2) Physical evaluation - Total ash value,
Acid insoluble ash, Water soluble ash and Moisture content. By using above parameters, finding the variation among three
nilavembu products

According to the size of the bacteria they are very small but the use of bacteria in different field of the world is unbelievable. The application of microbes is not for specific things. Its use is beyond what we can imagine. Currently our research is pointing out the use of bacteria in the healing of the crack of the concrete. Concrete is one of the most common materials using in the construction. Concrete is susceptible to external factors and in future we may have to face many calamities too. So there will be chances of formation of cracks and also the strength of the concrete may be lessening. The cracks formed in concrete are inescapable and they are the major reason for the weaknesses of the concrete. So the objective of the present research is to heal the concrete by the addition of the calcium carbonate precipitating bacteria that is Bacillus subtilis. This bacteria is mixed along with the concrete. Tests on the bacterial concrete by comparing with the standard concrete cube. The compression strength of both the cubes will be calculated and the calcium carbonate precipitation will also examine. By testing the compressive strength we can understand whether the bacteria have the ability to precipitate calcium carbonate, only if the calcium carbonate is precipitated the concrete will be stronger. Then only we can state that calcium carbonate is healing the crack. When the concrete is harden, cracks are healed by bacterial reaction as water ingresses through the cracks. It is a novice technique with an old perspective that the microbial mineral sedimentation persistently takes place naturally in the environment

The organic phase concentration required for the mobile phase can be estimated by gradient elution method. For aqueous sample mixtures, the best way to start is with gradient reversed phase chromatography. Gradient can be started with 10% organic phase in the mobile phase and organic phase concentration (Methanol & Acetonitrile) can be increased up to 100% within 20-60 min. Separation can then be optimised by changing the initial mobile phase composition and the slope of gradient according to the chromatogram obtained from preliminary run. The initial mobile phase composition can be estimated on the basis of where the compounds of interest were eluted, namely, at what mobile with composition. Changing the polarity of the mobile phase can alter elution of drug molecules. The elution strength of a mobile phase depends upon its polarity, the stronger the polarity, higher is the elution. Ionic samples (acidic or basic) can be separated, if they are present in un-dissociated form. Dissociation of ionic samples may be suppressed by proper selection of pH