Glaucoma is a condition that damage optic nerve and gets worse over time. It increases the pressure inside your eye. Glaucoma tends to be inherited and may not show up until later in life. The increased intraocular pressure, can damage the optic nerve. Without treatment, glaucoma can cause total permanent blindness within a few years. The problem arises with conventional dosage forms can be overcome by formulating ocular inserts. In this study atenolol is used as model drug. Atenolol is a beta blocker, and it exhibits physico-chemical properties similar to timolol maleate. In this study the influence of various polymers like hydroxypropyl methylcellulose and polyvinyl alcohol in different concentrations on drug release kinetics was studied. The data were subjected to pharmaco-kinetic analysis and various physical characteristics of films were evaluated. In vitro release revealed that formulation HPMC4 followed perfect zero order kinetics release. It was also observed that the release of drug increases with concentration of polymer up to an optimum concentration. From the present work, it was concluded that this ocular insert can be an innovative and promising approach for the delivery of atenolol with improved bioavailability, enhanced drug release with better patient compliance and as an effective therapy for the treatment of glaucoma. Keywords: Glaucoma, Atenolol, Beta blocker, Ocular insert, Hydroxypropyl methylcellulose, Polyvinyl alcohol.

A rapid and precise reverse phase high performance liquid chromatographic method has been developed for the validated of Velpatasvir and Sofosbuvir, in its pure form as well as in tablet dosage form. Chromatography was carried out on a Hypersil C18 (4.6 x 250mm, 5μm) column using a mixture of Acetonitrile: Water (50:50% v/v) as the mobile phase at a flow rate of 0.9ml/min, the detection was carried out at 235nm. The retention time of the Velpatasvir and Sofosbuvir was 2.079, 4.045 min respectively. The method produce linear responses in the concentration range of 5-25μg/ml for Velpatasvir, and 20-100μg/ml of Sofosbuvir. The method precision for the determination of assay was below 2.0%RSD. The method is useful in the quality control of bulk and pharmaceutical formulations. Keywords:Velpatasvir, Sofosbuvir, RP-HPLC, PDA Detection, Validation.

ABSTRACT Herbal Adhesive Bandages present in market were having Antiseptic property only and The present study aimed to design, develop, and evaluate the antiseptic as well as hemostatic (astringent) activity of herbal wound pad containing powdered herbal drug’s i.e. Tagetes erecta (marigold), Curcuma longa (Turmeric) and Acacia catechu (Kattha) The plants have been reported in the literature as having good antimicrobial, anti-inflammatory and Hemostatics (Astringent) Activity. Various formulation batches of herbal ointment were prepared and evaluated for various parameters like colour, appearance, pH, Consistency, Spreadability, Grittiness and antimicrobial activity. Prepared ointment was used for the preparation of zenduplast. The formulation of Batch# F4 was compared with the marketed preparation Clindamycin ointment. It is a very good attempt to establish the herbal antiseptic wound pad containing powders of Tagetes erecta, Curcuma longa and Acacia catechu. Zenduplast wound pad was successfully designed and developed after extensive manufacturing and evaluation process by specialized techniques for evaluation of antiseptic activity in vitro. Keywords: Tagetes erecta, Curcuma longa , Acacia catechu, Zenduplast, Antiseptic, Hemostatic.

Tuberculosis is the leading cause of death worldwide. With the emergence of powerful strains of causative bacteria Mycobacterium tuberculosis, there is a need to proactively discover and develop new leads on existing or newer targets. In this work, we present the newer 2,5-diaryl-1,3,4-Oxadiazole analogues and analyze in-silico binding to the shikimate kinase protein. In Mycobacterium tuberculosis shikimate kinase is essential for the viability of the pathogen. The shikimate kinase is involved in the phosphorylation of shikimate to shikimate-3-phosphate utilizing the ATP and forming ADP as a byproduct. The docking studies revealed that these compounds interacted with shikimate kinase enzyme with the residues, through hydrogen bonding of Lys15, Arg 58A, Gly80A, Gly81A, Arg 117A, hydrophobic interaction of Gly79, Gly80A, Gly81A, Arg117A, Asp34 and pi-stacking interaction of Phe49 and Phe57 determined at resolution of 1.8 Å. Targeted molecules were synthesized using reported methods, then purified and characterized using spectroscopic techniques. The compounds, 2-((1H-1,2,4-triazol-1-yl)methyl)-5-(2-bromophenyl)-1,3,4-oxadiazole and 2-((1H-imidazol-1-yl)methyl)-5-(2-bromophenyl)-1,3,4-oxadiazole, showed promising results as they had positive anti tubercular activity. Keywords: Shikimate kinase, oxadiazole synthesis, 2,5- diaryl-1,3,4-oxadiazole, Tuberculosis.

Orally Fast dissolving film (OFDF) is a dosage form which placed in the oral cavity, quickly gets hydrated, sticks onto the site of application and then disintegrates to release the drug. Agomelatine is an antidepressant also used for mood disorders such as anxiety and obsessive compulsive disorder. Fast dissolving films of Agomelatine were prepared by solvent casting technique. HPMC E-15 was selected as polymer because of its good water solubility. Polyvinyl pyrrolidone K-30(PVP K-30) as superdisintegrant and polyvinyl alcohol as film forming agent. Mannitol as sweetner and saliva stimulating agent used in the formulation. The compatibility of the drug in the formulation was confirmed by FTIR studies. A various concentration of polymers was used in order to optimize API concentration of the new dosage form. The orally fast dissolving film was characterized for weight, thickness, folding endurance, tensile strength and dissolution using In-vitro experimentations. The effect of PVA and PVP K-30 on drug release profile and film forming properties was investigated. Estimation of drug content of films was performed and the results were satisfactory. In-vitro dissolution studies revealed higher drug release from formulation F6 batch. Keywords:Orally fast dissolving film, Agomelatine, PVP K-30, PVA, Antidepressant.

Doxylamine succinate and Pyridoxine HCL was carried out by RP-HPLC method on a Symmetry C18 (4.6 x 150mm, 5mm, Make: Waters) using a mobile phase consisting of pH 3.5 phosphate buffer: Acetonitrile (30:70). The mobile phase was pumped at a rate of 1.0 ml/min and the detection was carried out at 254nm. The retention time of Doxylamine succinate and Pyridoxine HCL was found to be 2.162 and 3.305 min respectively and linearity was in the range of 12-60µg /ml for Pyridoxine HCL and 20-100µg/ml for Doxylamine succinate. The results obtained in newer RP-HPLC method for determination of Doxylamine succinate and  Pyridoxine HCL are tabulated and  also discussed about the developed RP-HPLC method. The proposed method is simple cost effective and gives reliable assay results with short analysis time (5min). The content of drugs in the formulation was found to be 60mg Pyridoxine and 100mg Doxylamine succinate.The method was validated  in terms of sensitivity, accuracy and precision and can be used for the routine determination of Doxylamine succinate and  Pyridoxine HCL in pharmaceutical formulations. The above method does not suffer from any interference due to common excipients. Therefore the proposed RP-HPLC method could be successfully applied to estimate commercial pharmaceutical products containing Doxylamine succinate and Pyridoxine HCL.

Keywords: Symmetry C18, Doxylamine succinate and Pyridoxine, RP-HPLC.